Diclofenac improved the efficacy 
of lomefloxacin in the aqueous humor

Stara Sagora – mechentel news – The results of the Study from S.Z.Krustev et al. from the Department of Veterinary Surgery, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria to evaluate thre Effect of diclofenac on ocular levels of ciprofloxacin and lomefloxacin in rabbits with endophthalmitis, show that Topical administration of lomefloxacin and diclofenac in combination improved penetration of the antibacterial agent in the aqueous humor which can be of clinical importance. To analyze ciprofloxacin and lomefloxacin penetration into the anterior eye tissues after topical instillation in healthy rabbits and with experimental Staphylococcus aureus endophthalmitis, additionally, effect of diclofenac sodium eye drops on the distribution of both fluoroquinolones in the inflamed eye tissues was investigated. An intense protocol with frequent antibiotic administration was chosen. Samples from aqueous humor were obtained 2 and 6 h after the start of the treatment. Samples from cornea and iris were obtained at the end of the experiment, after euthanasia of the animals. Drug concentrations were measured by HPLC method. The median levels of ciprofloxacin and lomefloxacin in aqueous humor of healthy animals, 2 and 6 h after drug administration were 6.39-9.65 and 5.30-6.81 µg/ml, respectively. Ciprofloxacin levels were neither changed from the inflammation nor after instillation of diclofenac. In contrary, lomefloxacin concentrations in aqueous humour of inflamed eye were significantly increased 12.15-15.08 µg/ml, especially after diclofenac administration (17.12-27.76 µg/ml). Levels of both fluoroquinolones in cornea (13.08 µg/g for ciprofloxacin and 12.25 µg/g for lomefloxacin) and in iris (0.84 µg/g for ciprofloxacin and 1.34 µg/g for lomefloxacin) were higher than MIC and MBC values against S. aureus ATCC 25923. Published in the August issue of the journal Drug Dev Ind Pharm, the authors : “higher lomefloxacin concentrations were observed in the aqueous humor after instillation of diclofenac, the levels of both fluoroquinolones in iris and in cornea were not significantly changed”

Autoren: Krustev SZ, Rusenova NV, Haritova AM. Korrespondenz: Department of Veterinary Surgery, Department of Veterinary Microbiology, Infectious and Parasitic Diseases, and Department of Pharmacology, Physiology of Animals and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria. Studie: Effect of diclofenac on ocular levels of ciprofloxacin and lomefloxacin in rabbits with endophthalmitis. Quelle: Drug Development and Pharmacy, 2013 Aug 13 doi:10.3109/03639045.2013.828225. http://informahealthcare.com/doi/abs/10.3109/03639045.2013.828225